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A Volcano plots showing the differential genes in metastatic versus non-metastatic OS tissues in GSE21257 . B GO functional enrichment analysis of significantly differential genes. C The acetyl coenzyme A biosynthetic process in GSEA analysis. D Pattern diagram of acetyl coenzyme A biosynthetic process. E Pulmonary metastasis-free survival analysis of different ACSS1 expression in OS patients. F Immunohistochemical analysis of ACSS1 expression in specimens from patients with OS. G Immunofluorescence analysis showing the localization of ACSS1 in OS cells. H Protein expression of ACSS1 in OS cell lines and <t>HMSC.</t>
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A Volcano plots showing the differential genes in metastatic versus non-metastatic OS tissues in GSE21257 . B GO functional enrichment analysis of significantly differential genes. C The acetyl coenzyme A biosynthetic process in GSEA analysis. D Pattern diagram of acetyl coenzyme A biosynthetic process. E Pulmonary metastasis-free survival analysis of different ACSS1 expression in OS patients. F Immunohistochemical analysis of ACSS1 expression in specimens from patients with OS. G Immunofluorescence analysis showing the localization of ACSS1 in OS cells. H Protein expression of ACSS1 in OS cell lines and <t>HMSC.</t>
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A Volcano plots showing the differential genes in metastatic versus non-metastatic OS tissues in GSE21257 . B GO functional enrichment analysis of significantly differential genes. C The acetyl coenzyme A biosynthetic process in GSEA analysis. D Pattern diagram of acetyl coenzyme A biosynthetic process. E Pulmonary metastasis-free survival analysis of different ACSS1 expression in OS patients. F Immunohistochemical analysis of ACSS1 expression in specimens from patients with OS. G Immunofluorescence analysis showing the localization of ACSS1 in OS cells. H Protein expression of ACSS1 in OS cell lines and <t>HMSC.</t>
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A Volcano plots showing the differential genes in metastatic versus non-metastatic OS tissues in GSE21257 . B GO functional enrichment analysis of significantly differential genes. C The acetyl coenzyme A biosynthetic process in GSEA analysis. D Pattern diagram of acetyl coenzyme A biosynthetic process. E Pulmonary metastasis-free survival analysis of different ACSS1 expression in OS patients. F Immunohistochemical analysis of ACSS1 expression in specimens from patients with OS. G Immunofluorescence analysis showing the localization of ACSS1 in OS cells. H Protein expression of ACSS1 in OS cell lines and <t>HMSC.</t>
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A Volcano plots showing the differential genes in metastatic versus non-metastatic OS tissues in GSE21257 . B GO functional enrichment analysis of significantly differential genes. C The acetyl coenzyme A biosynthetic process in GSEA analysis. D Pattern diagram of acetyl coenzyme A biosynthetic process. E Pulmonary metastasis-free survival analysis of different ACSS1 expression in OS patients. F Immunohistochemical analysis of ACSS1 expression in specimens from patients with OS. G Immunofluorescence analysis showing the localization of ACSS1 in OS cells. H Protein expression of ACSS1 in OS cell lines and <t>HMSC.</t>
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A Volcano plots showing the differential genes in metastatic versus non-metastatic OS tissues in GSE21257 . B GO functional enrichment analysis of significantly differential genes. C The acetyl coenzyme A biosynthetic process in GSEA analysis. D Pattern diagram of acetyl coenzyme A biosynthetic process. E Pulmonary metastasis-free survival analysis of different ACSS1 expression in OS patients. F Immunohistochemical analysis of ACSS1 expression in specimens from patients with OS. G Immunofluorescence analysis showing the localization of ACSS1 in OS cells. H Protein expression of ACSS1 in OS cell lines and <t>HMSC.</t>
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A Volcano plots showing the differential genes in metastatic versus non-metastatic OS tissues in GSE21257 . B GO functional enrichment analysis of significantly differential genes. C The acetyl coenzyme A biosynthetic process in GSEA analysis. D Pattern diagram of acetyl coenzyme A biosynthetic process. E Pulmonary metastasis-free survival analysis of different ACSS1 expression in OS patients. F Immunohistochemical analysis of ACSS1 expression in specimens from patients with OS. G Immunofluorescence analysis showing the localization of ACSS1 in OS cells. H Protein expression of ACSS1 in OS cell lines and HMSC.

Journal: Journal of Orthopaedic Translation

Article Title: Acetyl-CoA synthase 1 mediates metabolic reprogramming to promote proliferation and metastasis of osteosarcoma ☆ ☆☆

doi: 10.1016/j.jot.2026.101052

Figure Lengend Snippet: A Volcano plots showing the differential genes in metastatic versus non-metastatic OS tissues in GSE21257 . B GO functional enrichment analysis of significantly differential genes. C The acetyl coenzyme A biosynthetic process in GSEA analysis. D Pattern diagram of acetyl coenzyme A biosynthetic process. E Pulmonary metastasis-free survival analysis of different ACSS1 expression in OS patients. F Immunohistochemical analysis of ACSS1 expression in specimens from patients with OS. G Immunofluorescence analysis showing the localization of ACSS1 in OS cells. H Protein expression of ACSS1 in OS cell lines and HMSC.

Article Snippet: Human bone marrow mesenchymal stem cells (HMSC) were derived from ATCC (Manassas).

Techniques: Functional Assay, Expressing, Immunohistochemical staining, Immunofluorescence